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Nirosha (Narosha/Nirosha) Murugan (guest/researcher)

👤 Person
314 appearances

Podcast Appearances

Radiolab
The Spark of Life

You certainly are.

Radiolab
The Spark of Life

I know how to use these.

Radiolab
The Spark of Life

I'm Narosha Murugan, an applied biophysicist from Waterloo, Canada.

Radiolab
The Spark of Life

And most biophysicists look at mostly bio.

Radiolab
The Spark of Life

I'm on the other end, who likes to be 50-50.

Radiolab
The Spark of Life

I mean, I can tell you a very specific moment in grad school that... Tell me.

Radiolab
The Spark of Life

When I was living in the dorms and I was making mashed potatoes and I burnt myself.

Radiolab
The Spark of Life

And then, I don't know why I thought this, but I thought it was really exciting.

Radiolab
The Spark of Life

How quickly that information of me burning my hand went into my body for me to remove my hand.

Radiolab
The Spark of Life

Like that signal had to go up my arm.

Radiolab
The Spark of Life

Things had to change and move all the way back down my arm for me to remove it.

Radiolab
The Spark of Life

Think of the molecular interactions.

Radiolab
The Spark of Life

And suddenly it just seemed impossible.

Radiolab
The Spark of Life

When we think about a protein, proteins have a very specific shape, and that shape determines their function.

Radiolab
The Spark of Life

So when you think of a cell doing what it needs to do, on the surface of a cell there are other proteins, which is what we call receptors.

Radiolab
The Spark of Life

And those receptors have a shape to them.

Radiolab
The Spark of Life

And for them to interact, there needs to be a physical interaction of that protein into the receptor.

Radiolab
The Spark of Life

It's as simple.

Radiolab
The Spark of Life

That's the fundamental basis of biomolecular interactions.

Radiolab
The Spark of Life

But thinking about all that for that one specific molecule to find that perfect receptor just seemed like it was too easy.

Radiolab
The Spark of Life

So that's exactly, that's the model that didn't sit well with me.

Radiolab
The Spark of Life

So imagine you're one of those like janitors with like a big ring of keys.

Radiolab
The Spark of Life

How do you find that right key for the right lock in that right amount of time to induce signaling?

Radiolab
The Spark of Life

You got to go through all those keys.

Radiolab
The Spark of Life

You got to try, iterate through random probability and get the right shape in the right space.

Radiolab
The Spark of Life

This is what I was like uncomfortable with is...

Radiolab
The Spark of Life

Think of that time and think of the probability of you finding your shape in one thousandth of a second.

Radiolab
The Spark of Life

And that's just like one interaction.

Radiolab
The Spark of Life

And so if you kind of break it down that way, and that's what I learned in school, things weren't adding up.

Radiolab
The Spark of Life

There's something missing.

Radiolab
The Spark of Life

There had to be something else to induce signaling inside of a cell.

Radiolab
The Spark of Life

So my advanced immunology teacher in grad school, I, after class, went up to him and I was like, well, how does that lock and key model make sense?

Radiolab
The Spark of Life

Think of the time and the probability.

Radiolab
The Spark of Life

And I asked him that question.

Radiolab
The Spark of Life

And he said, I don't know, but this is how it works.

Radiolab
The Spark of Life

And I'm like, no, but how?

Radiolab
The Spark of Life

Like, you know, I'm that annoying grad student.

Radiolab
The Spark of Life

But like, can you tell me a little bit more?

Radiolab
The Spark of Life

Like, where does the time fit in?

Radiolab
The Spark of Life

And he said, this is just the way it is.

Radiolab
The Spark of Life

I don't know how it works.

Radiolab
The Spark of Life

And that I don't know...

Radiolab
The Spark of Life

was enough for me to figure out maybe I can go find out that I don't know.

Radiolab
The Spark of Life

The gap that I was trying to fill is that how can the chemistry, how can the physical interactions occur so quickly?

Radiolab
The Spark of Life

Why can't we have the same thing, but through non-physical interactions?

Radiolab
The Spark of Life

So the way that I kind of like picture it is maybe if you had a door with a tap card access versus an actual old school locking key, you can open the door both ways.

Radiolab
The Spark of Life

Either the proteins can do it, that will take a longer time to do the behavior, or the

Radiolab
The Spark of Life

Like a wireless tap where you can just kind of put a card against a key receiver and there's a signal or a door opens.

Radiolab
The Spark of Life

So, okay, what can be faster that cells can use to communicate?

Radiolab
The Spark of Life

What is the fastest signal that we know?

Radiolab
The Spark of Life

It is the fastest modality that exists in our universe.

Radiolab
The Spark of Life

And then you go out, or what I did is went out to research to see if anyone else has asked those questions and how they test them.

Radiolab
The Spark of Life

And through my research, I found the original papers that showed that biology emits light.

Radiolab
The Spark of Life

Biology emits light?

Radiolab
The Spark of Life

It was the very first instance that someone thought, hey, biology emits light.

Radiolab
The Spark of Life

That was the first experiment.

Radiolab
The Spark of Life

What we now know is every cell in your body does give off light.

Radiolab
The Spark of Life

And the how kind of comes down to the part of the cell that's actually giving off light, which is involved with metabolism.

Radiolab
The Spark of Life

So if anything can metabolize, plants give off light.

Radiolab
The Spark of Life

Shrimp give off light.

Radiolab
The Spark of Life

Literally everything that is alive emits light.

Radiolab
The Spark of Life

So I'm glowing right now.

Radiolab
The Spark of Life

You're glowing right now.

Radiolab
The Spark of Life

Why can't I see it?

Radiolab
The Spark of Life

Because that's a good question.

Radiolab
The Spark of Life

Finally, we get to one.

Radiolab
The Spark of Life

No, that's fantastic.

Radiolab
The Spark of Life

We physically can't see it.

Radiolab
The Spark of Life

It's because the intensity of light is so weak.

Radiolab
The Spark of Life

And, you know, it has to come and go through all this tissue to come outside so that we could see it.

Radiolab
The Spark of Life

And so if you take a cell in a dish, any cell in a dish, it will give off light.

Radiolab
The Spark of Life

And we now know very confidently that it's wavelength specific.

Radiolab
The Spark of Life

What does that mean?

Radiolab
The Spark of Life

Different rates of metabolism will induce different wavelengths of light, so in different colors.

Radiolab
The Spark of Life

So the intensity of light is definitely not as bright as some of the aura pictographs that you might see.

Radiolab
The Spark of Life

For us to detect it, we have to have an ultra dark room and use these high sensitive detectors to even detect one photon.

Radiolab
The Spark of Life

So let's dig into that a little bit.

Radiolab
The Spark of Life

We know that light gets emitted from cells.

Radiolab
The Spark of Life

The question now is, where exactly is it coming from?

Radiolab
The Spark of Life

That's the question that I get all the time.

Radiolab
The Spark of Life

What's the mechanism?

Radiolab
The Spark of Life

My hypothesis is that most of it kind of comes down to the mitochondria.

Radiolab
The Spark of Life

So in that process, the electron goes from a high energy all the way down to a low energy state.

Radiolab
The Spark of Life

That's one way to look at it.

Radiolab
The Spark of Life

So during that hop, it releases energy, which is light.

Radiolab
The Spark of Life

If the electron doesn't make it, there should be no light, right?

Radiolab
The Spark of Life

That's the logic.

Radiolab
The Spark of Life

And that's what we're starting to find.

Radiolab
The Spark of Life

So when we've measured it.

Radiolab
The Spark of Life

So if you take a dish of brain cells from a rat, if it's just at rest, just doing nothing, really, you probably get around 100 photons a second.

Radiolab
The Spark of Life

When you add a dish of brain cells, like how many brain cells?

Radiolab
The Spark of Life

As a group, yeah.

Radiolab
The Spark of Life

And then when you activate them, we get signals anywhere from 1,000 to 2,000 photons a second.

Radiolab
The Spark of Life

Why did I not learn about this?

Radiolab
The Spark of Life

That's an excellent question.

Radiolab
The Spark of Life

That's something that I would like to change.

Radiolab
The Spark of Life

I think there's a lot of resistance to trying to understand this.

Radiolab
The Spark of Life

About like 10 years ago when I first started this stuff, I had my first...

Radiolab
The Spark of Life

When I presented this as a graduate student at a conference, it was awful.

Radiolab
The Spark of Life

Wait, what happened?

Radiolab
The Spark of Life

I presented our first, because I was really excited about this, so I wanted to incorporate this into my graduate thesis.

Radiolab
The Spark of Life

And, oh boy, did I get it.

Radiolab
The Spark of Life

Oh, this is noise.

Radiolab
The Spark of Life

This is not science.

Radiolab
The Spark of Life

You're going to jeopardize your career.

Radiolab
The Spark of Life

Go back into cell biology.

Radiolab
The Spark of Life

I think there's a lot of that.

Radiolab
The Spark of Life

But within the last decade, it's not just me.

Radiolab
The Spark of Life

There's several other researchers across the globe.

Radiolab
The Spark of Life

So now there's an acceptance of, OK, we'll believe it.

Radiolab
The Spark of Life

There's light coming off of biology.

Radiolab
The Spark of Life

Now the resistance is, okay, we accept that there's light coming off, but it's noise.

Radiolab
The Spark of Life

It's not meaningful light that's used in biology.

Radiolab
The Spark of Life

So what I'm looking into now is, okay, light's being generated from the mitochondria.

Radiolab
The Spark of Life

Does that light carry some form of information that the cell can use to do what it needs to do?

Radiolab
The Spark of Life

Is it purposeful and then being utilized by the body?

Radiolab
The Spark of Life

So I was thinking, okay, we're sitting in a bath of light that's coming from this big ball of fire, which we call the sun.

Radiolab
The Spark of Life

Does this light have any impact on our physiology?

Radiolab
The Spark of Life

Like, you know, okay, before I understand internal light,

Radiolab
The Spark of Life

What does external light do?

Radiolab
The Spark of Life

You were just like, how are we interacting with external light?

Radiolab
The Spark of Life

Does any of that apply to the internal light we're making?

Radiolab
The Spark of Life

The light's the same.

Radiolab
The Spark of Life

The photon is the same.

Radiolab
The Spark of Life

For example, there are these proteins called opsins in your eyes that help convert different wavelengths of light that help regulate circadian rhythms.

Radiolab
The Spark of Life

I guess that makes sense to me because the eyeball is a light element.

Radiolab
The Spark of Life

I mean, if you go to any literature, the first thing that will come up is vitamin D synthesis.

Radiolab
The Spark of Life

It's that, okay, the sun...

Radiolab
The Spark of Life

And it hits your skin and your skin processes vitamin D and, you know, that does a lot of things for metabolism.

Radiolab
The Spark of Life

So you're saying like my skin is working with the sun.

Radiolab
The Spark of Life

So like sun hits me.

Radiolab
The Spark of Life

And then what does my body do?

Radiolab
The Spark of Life

The vitamin D precursors absorb a certain wavelength from the sun.

Radiolab
The Spark of Life

They're absorbing wavelengths.

Radiolab
The Spark of Life

Wavelength, yeah.

Radiolab
The Spark of Life

There's information in that light.

Radiolab
The Spark of Life

And they convert shape.

Radiolab
The Spark of Life

That shape is what we can absorb.

Radiolab
The Spark of Life

Oh my God, I never thought of us as so plant-like.

Radiolab
The Spark of Life

Yeah, we are essentially like energetic converters converting sunlight into energy for our life.

Radiolab
The Spark of Life

We have light receptors in our brain.

Radiolab
The Spark of Life

Seems so dark in there.

Radiolab
The Spark of Life

Our pigment cells, like, you know, the melanocytes, the hemoglobin that carries the oxygen within your red blood cell absorbs light.

Radiolab
The Spark of Life

There are a lot more and we're starting to see more and more as people start to look at interactions with light, we can see that molecules have inherent abilities to absorb light.

Radiolab
The Spark of Life

Yeah, that's a good question.

Radiolab
The Spark of Life

And we don't know.

Radiolab
The Spark of Life

My hypothesis is that the cell generating light is purposeful, but we don't have the evidence to strongly say yes or no.

Radiolab
The Spark of Life

Those are all next steps.

Radiolab
The Spark of Life

So I think, yeah, there's some like a lot more questions to be asked.

Radiolab
The Spark of Life

I mean, and then the next question, if this light that's coming off of these mitochondria, if it's purposeful...

Radiolab
The Spark of Life

How is it getting from point A to point B in the cell?

Radiolab
The Spark of Life

Photons scatter, right?

Radiolab
The Spark of Life

So like photons, when it gets released, it's not like I'm going this direction.

Radiolab
The Spark of Life

It'll be scattered.

Radiolab
The Spark of Life

I'm not going north.

Radiolab
The Spark of Life

I'm just an explosion of photons.

Radiolab
The Spark of Life

And if we are going to say that it's purposeful, it needs to be guided into a destination.

Radiolab
The Spark of Life

So then your question is, how does it get from A to B without going off course in a photon-like manner?

Radiolab
The Spark of Life

And what is the biology that would support that?

Radiolab
The Spark of Life

And there is some evidence suggesting that maybe the cytoskeleton is a means to guide photons.

Radiolab
The Spark of Life

It's the skeleton, the scaffolding of the cell.

Radiolab
The Spark of Life

It's specifically made up of various proteins.

Radiolab
The Spark of Life

And one of our interests within this scaffold is called microtubules.

Radiolab
The Spark of Life

that form in a long rod-like structure.

Radiolab
The Spark of Life

They're the ones that help create that shape of the cell.

Radiolab
The Spark of Life

Because if we look at images of a cell, you can actually see mitochondria really, really close to the cytoskeletal rods.

Radiolab
The Spark of Life

And they get moved along the cytoskeleton, like little train tracks to physically move within cells.

Radiolab
The Spark of Life

Oh, mitochondria themselves will attach on to these microtubules and

Radiolab
The Spark of Life

I mean, and that's how things move within the cell.

Radiolab
The Spark of Life

It's not just like random blobs floating around.

Radiolab
The Spark of Life

I mean, it sounded like everything was floating.

Radiolab
The Spark of Life

That's so cute.

Radiolab
The Spark of Life

And like things hop on and off.

Radiolab
The Spark of Life

They're called kinesins and dinesins, but...

Radiolab
The Spark of Life

I'm going to stick with tram.

Radiolab
The Spark of Life

But so, you know, if the mitochondria are in close proximity to these railway tracks or these microtubules, the light that's being emitted could be absorbed by that microtubule and be propagated down that track.

Radiolab
The Spark of Life

Like a fiber optic cable.

Radiolab
The Spark of Life

And so what we're testing right now is a series of experiments to see if the microtubule is that biological fiber optic cable.

Radiolab
The Spark of Life

We are working on that currently right now, but we do have strong evidence to show that the light that's being generated from neural cells, your brain cells, they are not random, that they are tied to purposeful activity of those neurons.

Radiolab
The Spark of Life

Well, it's the same kind of question we can, what kind of information does electricity carry?

Radiolab
The Spark of Life

I don't know, Roshan.

Radiolab
The Spark of Life

I just ask the questions.

Radiolab
The Spark of Life

I'm not... No, no.

Radiolab
The Spark of Life

But the information in this case is that the fact that, you know, maybe the wavelength, the oscillations of these light, the fact that they could carry biological information itself would be meaningful because if you look into your brain, between your two brain cells or, you know, things that carry information from one part of your brain to the other, we call that the white matter or the axons.

Radiolab
The Spark of Life

The white matter we're starting to see can carry photons.

Radiolab
The Spark of Life

So maybe each of those bundles of nerves act like a fiber optic cable.

Radiolab
The Spark of Life

And the same fiber optic cable that we see in telecommunication, they carry pulses of light that we use to carry information.

Radiolab
The Spark of Life

Why can't our brain do that?

Radiolab
The Spark of Life

And this could be like memories.

Radiolab
The Spark of Life

Is it like signals?

Radiolab
The Spark of Life

Why can't a thought be transported in the form of light?

Radiolab
The Spark of Life

And it kind of, you know, if we're really thinking far ahead, are these photons involved in trying to help us understand consciousness?

Radiolab
The Spark of Life

What myself and a few other people are doing is, the photons are there.

Radiolab
The Spark of Life

Can we use it to discriminate between things?

Radiolab
The Spark of Life

if they're at the very least tied to metabolism, are they photonic biomarkers?

Radiolab
The Spark of Life

Like, can I say, I know that's a heart.

Radiolab
The Spark of Life

I know that's a tumor.

Radiolab
The Spark of Life

I know that's a kidney.

Radiolab
The Spark of Life

At the very least, can we do that?

Radiolab
The Spark of Life

And so what I'm trying to do is use that for cancer.

Radiolab
The Spark of Life

So for cancer use, we know that cancer have dysfunctional mitochondria or non-normal mitochondria.

Radiolab
The Spark of Life

So from there, can you imagine if when at the very beginning inception, we can pick up that early change as soon as they happen, as soon as they're different from their healthy counterparts.

Radiolab
The Spark of Life

If we can pick up that using photons, that means we can pick up cancer as early as the inception point.

Radiolab
The Spark of Life

We don't need to have an accumulation of molecules and mutations.

Radiolab
The Spark of Life

We don't have to wait that long.

Radiolab
The Spark of Life

Yeah, you need like a whole tumor.

Radiolab
The Spark of Life

Yeah, you need a sizable mass, basically.

Radiolab
The Spark of Life

Yes, with confidence I can say this.

Radiolab
The Spark of Life

We've published papers on this now.

Radiolab
The Spark of Life

So we can tell whether there is cancer within an animal as early as that we've injected it.

Radiolab
The Spark of Life

So in these experiments, we'll take a rat and we have injected underneath its skin melanoma.

Radiolab
The Spark of Life

And on day one after injection, and we did this in a double blind way where a grad student has come with detectors to look at animals that were injected versus not injected.

Radiolab
The Spark of Life

You can tell within day one that there's something, there's cancer there.

Radiolab
The Spark of Life

Is letting off photons.

Radiolab
The Spark of Life

And there was a paper that was published that showed that you can tell when an animal is alive and dead just by looking at their photon signatures.

Radiolab
The Spark of Life

This is the question I want to ask you.

Radiolab
The Spark of Life

So in that paper, I think the initial study was to just look at these different kinds of detectors when an animal was alive and dead.

Radiolab
The Spark of Life

And the photon signatures obviously dissipate when the animal dies.

Radiolab
The Spark of Life

What that study didn't look at, which you alluded to, is when.

Radiolab
The Spark of Life

When in that timescale does this signature end?

Radiolab
The Spark of Life

And that would be really cool.

Radiolab
The Spark of Life

When does the glow stop when you're dead?

Radiolab
The Spark of Life

For example, in hospice care, people report this death flash.

Radiolab
The Spark of Life

I originally heard about this when I went to a consciousness conference and there was this cardiothoracic surgeon.

Radiolab
The Spark of Life

He would say that he's seen it or his staff in the OR has seen this very sudden flash of light.

Radiolab
The Spark of Life

And I'm like, you have OR lights everywhere.

Radiolab
The Spark of Life

That's where I initially heard it and I looked into it a little bit.

Radiolab
The Spark of Life

And there's hospice nurses that have anecdotally mentioned this.

Radiolab
The Spark of Life

Yeah, yeah, yeah.

Radiolab
The Spark of Life

These are reports.

Radiolab
The Spark of Life

Are there any experimental evidence?

Radiolab
The Spark of Life

They're anesthesiologists.

Radiolab
The Spark of Life

Why would there be a big explosion of light you could suddenly see?

Radiolab
The Spark of Life

Well, when things die, there's a sudden release of these electrons.

Radiolab
The Spark of Life

They're not being propagated into certain proteins, right?

Radiolab
The Spark of Life

These electrons have nowhere to go.

Radiolab
The Spark of Life

And so when you have high energy photons dissipating, release is late.

Radiolab
The Spark of Life

So that's my hypothesis.

Radiolab
The Spark of Life

When the system, yeah, that goes back to like physics.

Radiolab
The Spark of Life

When there's no organization from biology, that energy has to go somewhere.

Radiolab
The Spark of Life

I think biology, these biomolecules, the membrane, all of these stuff inside of cells help organize that energy into meaningful processes.

Radiolab
The Spark of Life

So that's why I was saying way back when our beginning of our conversation is when we reframe our understanding of cells being these energetic bodies, I think the physical dimension makes a lot more sense.

Radiolab
The Spark of Life

Well, there's a really cool video that I can send to you where someone showed us the life flash.

Radiolab
The Spark of Life

As soon as a sperm enters the egg, there's a huge calcium influx.

Radiolab
The Spark of Life

Have you seen that video?

Radiolab
The Spark of Life

Wait, can I see this video?

Radiolab
The Spark of Life

Yeah, yeah, yeah.

Radiolab
The Spark of Life

You think it's just around?

Radiolab
The Spark of Life

You should be able to Google it.

Radiolab
The Spark of Life

Type in, I don't know, calcium life flash.